Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury

Table 3 Assay protocol modifications and summary of assay robustness results

Assay	Modification	Plasma pool	Replicate CV (%)	%recovery*
1	Standard protocol (Capture 1 h, Samples 2 h, Detection 1 h)	Low	3.7	–
		Intermediate	0.7	–
		High	6.7	–
2	Capture 30 min	Low	6.2	76.8
		Intermediate	3.7	89.4
		High	7.4	94.8
3	Capture overnight	Low	9.6	90.7
		Intermediate	7.1	115.5
		High	3.9	108.7
4	Samples 1 h	Low	3.4	101.7
		Intermediate	11.2	85.2
		High	9.7	97.9
5	Samples overnight	Low	18.2	72.7
		Intermediate	0.5	81.6
		High	2.0	80.4
6	Detection 30 min	Low	2.4	100.1
		Intermediate	2.3	110.8
		High	0.4	118.4

*%recovery = (concentration measured with modified assay protocol/concentration measured with standard assay protocol) × 100%. CV coefficient of variation

ISSN: 1758-9193